Luciferase and method for the determination of intracellular atp with the use of the same

摘要

A luciferase tolerant to surfactants; and a method for assaying intracellular ATP which comprises the first step of extracting ATP from a cell-containing sample in the presence of a surfactant, the second step of adding a luciferase-containing luminescent reagent to the ATP extract thus inducing luminescence and the third step of detecting the luminescence dose, characterized by using the luciferase tolerant to surfactants.