Optimization of TAT-peptide (TATp) Quantity on the Liposome Surface for Effective Intracellular Delivery of TATp-Conjugated PEG-Liposomes

摘要

TATp-PEG-PE was synthesized by reacting PE-PEG_(1,000)-Maleimide with TATp-SH, and rhodamine-labeled liposomes with varying quantity of the surface-associated TATp (from 0.25 to 5% mol %) were studied for the cell's uptake. The uptake of TATp-liposomes by cells significantly increased with the surface TATp density increase till approx. 2.5 mol % of TATp.