Detection method of binding events at the electrode microarray using an enzyme

摘要

The present invention provides a method for detecting binding events in the electrode microarray. Microarrays have a capture complex types of two or more at a site accessible electrodes and the corresponding electrode. Capture complexes to capture the analyte. I want to form a reporter complex by binding the enzyme. To produce a detectable enzyme product in the electrode due to the difference in the electrical response of the electrode without the enzyme product and electrodes can be contacted continuously substrate solution having an enzyme product. May be a solid deposition product enzyme product. By reading the electrical properties of the electrode on the microarray, to know the existence of the enzyme product by switching sequentially to earth the electrodes kept at a constant voltage, then returned to the constant voltage.