Quinacridone derivatives as fluorescence detection for the labeling reagent of biological material

摘要

New quinacridone dye derivative having a fluorescence lifetime characteristic is disclosed. In addition, it is also disclosed the use of marking materials in biological assays and methods of labeling a target biological material using a quinacridone pigment. Structure (I) [where, independently, Z ² Z ¹ and represents the atoms necessary one ring, to complete a heterocyclic aromatic or three fused aromatic or 2 fused quinacridone derivatives , carbon atoms, and having 5 or 6 atoms that are selected from the atoms of 2 or less by optionally selected from oxygen, nitrogen and sulfur, the ^{ring, R} 3, R 4, R 5, R ⁶ , independently, ^{R 8} and ^{R 7} is selected from the group consisting of hydrogen, halogen, amide, hydroxyl, cyano, nitro, mono - nitro - - or di-substituted benzyl, amino, mono - substituted amino - di _-C 1 _{-C 4} alkyl or , sulfhydryl, carbonyl, _carboxyl, C 1 -C ₆ alkoxy, acrylate, vinyl, styryl, aryl, _heteroaryl, C 1 _{-C 20} alkyl, aralkyl, sulphonate, sulphonic acid, quaternary ammonium, and based -E-F independently, is selected from _n Y, ^{R 2} and ^{R 1} is hydrogen, mono based - - (CH ₂₎ - nitro - - or di-substituted _benzyl, C 1 _{-C 20} alkyl, aralkyl, based -E-F and groups _- (CH 2 -) is selected from _{n Y,} E is a spacer group, F is a target bonding group, Y is selected from carboxyl sulfonate, sulfate, phosphonate, phosphate, quaternary ammonium, n has a] is an integer of 1 to 6. The present invention relates to a set of fluorescent quinacridone dye derivatives different, each dye is assumed to have a fluorescence lifetime different set of dye is particularly useful for multiparameter analysis.