Provided is a method of producing D-psicose using a D-psicose epimerase derived from Agrobacterium tumefaciens. Provided are a protein having an amino acid sequence of SEQ ID NO:1 and having a psicose 3-epimerase activity, a gene encoding the protein, a recombinant expression vector containing the gene, and a method of producing D-psicose by reacting the protein produced on a mass scale with D-fructose. The method of producing D-psicose is an environmentally friendly method using a new enzyme, in which an inexpensive substrate is used, and the activity of the enzyme can be retained for a prolonged time period. Thus, the method can be efficiently used for the mass production of D-psicose.
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机译:提供了一种使用衍生自根癌农杆菌的D-聚蔗糖差向异构酶生产D-聚蔗糖的方法。提供具有氨基酸序列SEQ ID NO:1的蛋白质和具有磷酸3-表异构酶活性的蛋白质,编码该蛋白质的基因,包含该基因的重组表达载体以及通过使该蛋白质反应而产生D-蛋白质的方法。用D-果糖大规模生产。生产D-庚糖的方法是使用新酶的环境友好方法,其中使用便宜的底物,并且可以延长酶的活性。因此,该方法可以有效地用于D-阿胶糖的大规模生产。
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