Detection quantitative method of wheat indwelling characteristic DNA, and decisive method of mixture ratio of the gene recombination wheat in the suffering inspection sample

摘要

Purpose of this invention a) exists universally in the wheat kind, the abundance (the detection quantity) is not inclined b) by the wheat kind, c) other grain exists and only the pan wheat is detection possible without intersection characteristic, you discover the wheat indwelling characteristic arrangement which satisfies the condition d) for being expanded quantitatively with the PCR reaction, you designate that the method where accurately it detects or measures wheat indwelling characteristic DNA in the suffering inspection sample, with the polymerase chain reaction compared to, or is offered as purpose. This invention wheat indwelling characteristic DNA in the suffering inspection sample, being the method where it detects or measures with the polymerase chain reaction, or, to designate the nuclear acid which it extracts from the nuclear acid or the aforementioned suffering inspection sample in the aforementioned suffering inspection sample as the mold, arrangement number: Process, and the description above which expand the nuclear acid of the aforementioned territory making use of the expansion possible primer pair which is included in the territory consisting of the base arrangement or that part arrangement of statement in 2 the nuclear acid which is expanded it offers the method of including the process which it detects or measures, or.