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Detection quantitative method of wheat indwelling characteristic DNA arrangement

机译:小麦残留特​​征DNA排列的检测定量方法

摘要

This invention specifies, with the single copy, at the same time in PCR the wheat indwelling characteristic DNA which can detect the wheat uniquely without intersection characteristic of the other plant (the genome) the part territory designates that the primer in order to expand this part territory and ideal detection quantitative method of the indwelling characteristic DNA which uses these are offered as purpose. This invention designates the nuclear acid which is extracted from the nuclear acid or the aforementioned suffering inspection sample in the aforementioned suffering inspection sample in the method where it detects or measures wheat indwelling characteristic DNA arrangement in the suffering inspection sample, with the polymerase chain reaction, or, as the mold, either of 7 the territory where at least 80% of base arrangement of statement is included in one process, and the description above which expand the nuclear acid of the aforementioned territory making use of expansion possible primer pair the nuclear acid which is expanded offers the method of including the process which it detects or measures, or from arrangement number 1.
机译:本发明以单拷贝的方式,在PCR中同时指定了可以在没有其他植物(基因组)的交叉特性的情况下唯一地检测小麦的小麦残留特​​征DNA,该部分区域指定用于扩展该部分的引物。目的是提供一种利用这些特征的内在特征DNA的领域和理想的检测定量方法。本发明是指通过聚合酶链反应检测或测定受害检查样品中的小麦残留特​​征DNA的排列的方法,从上述受害检查样品中的核酸或上述受害检查样品提取的核酸。或者,作为模子,在一个过程中包含至少80%陈述的基本安排的7个地区中的任何一个,以及上面的描述,其中所述核苷酸利用可扩展引物对扩展上述区域的核酸扩展后的方法提供了一种方法,该方法包括它检测或测量的过程,或从排列号1开始的过程。

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