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Oligonucleotide N3 '→ P5' and solid-phase synthesis of phosphoramidate
Oligonucleotide N3 '→ P5' and solid-phase synthesis of phosphoramidate
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机译:寡核苷酸N3'→P5'与氨基磷酸酯的固相合成
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摘要
(57) there is provided a method of using an amine-exchange reaction of the phosphoramidite, synthesizing oligonucleotide N3 '→ P5' phosphoramidate, where [Abstract] The present invention, the solid support oligonucleotide chain Deprotection 3'-amino group is replaced with an amino moiety of the incoming monomer having a protected 3'-amino group of the 5'-phosphoramidite. Then be oxidized, inter nucleoside phosphoramidite coupling obtained to form a stable protective phosphoramidate linkage. Synthesis than currently available methods of compounds of the above type, the method of the present invention reduces the amount of reagents, and greatly improves product yield.
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