R - hidroxinitrila liases that improve the acceptance of the substrate and the use of the same

摘要

R - hidroxinitrila liases that improve the acceptance of the substeato and the use of the same. R - hidroxinitrila liases showed an improvement in the acceptance of the substrate, increased activity and selectivity increased.These R - hidroxinitrila liases of rose family CEAE present a substitution at amino acid residue in the sequ00fau00eancia amino acid corresponding to position of the mature protein pahnl5 360 by another amino acid support or neutral amino acid and / or (b) the corresponding amino acid residue The position of pahnl5 mature protein 225 by polar amino acid, and it can also through the inclusion.When appropriate, from 1 to 20 residues in the active center or in the hydrophobic channel leading to the replacement of the active center.