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R-HYDROXYNITRILE LYASES HAVING IMPROVED SUBSTRATE ACCEPTANCE AND USE THEREOF
R-HYDROXYNITRILE LYASES HAVING IMPROVED SUBSTRATE ACCEPTANCE AND USE THEREOF
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机译:具有改进的基质可接受性的R-羟基腈裂解酶及其用途
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摘要
R-hydroxynitrile lyases having an improved substrate acceptance, increased activity and increased selectivity, in which there is replacement in the amino acid sequence of R-hydroxynitrile lyases from the Rosaceae family either a) of the amino acid residue which corresponds to position 360 of the mature PaHNL.5 protein by another apolar amino acid or a neutral amino acid and/or b) of the amino acid residue which corresponds to position 225 of the mature PaHNL5 protein by another polar amino acid, it also being possible where appropriate for 1 to 20 further residues in the active center or in the hydrophobic channel leading to the active center to be replaced.
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