AN ENZYME-LINKED IMMUNOSORBENT ASSAY PROCEDURE BY SONICATION

摘要

The present invention relates to a rapid enzyme-linked immunosorbent assay procedure for the detection of small quantities of biomolecule such as antigen or antibody by sonication. This invention particularly relates to the sonication -assisted enzyme-linked immunosorbent assay (Sono-ELISA or SELISA) which comprises of immobilization of antigen or antibody onto the solid surface and performing subsequent ELISA steps by sonication at a frequency ranging between 25 -130 kHz with the power output ranging from 60 W to 300 W for a time period ranging from 1 to 30 minutes and at a temperature ranging from 30°C to 60°C. The ELISA method described in the present invention can be achieved very fast preferably in the time range of 4 - 40 minutes. The invented procedure is useful for performing ELISA required in diagnostics, agriculture, food technology, environmental science, research work etc. The invented ELISA procedure is rapid, economical, reproducible and simple and has the potential for automation.