Actinobacillus succinogenes genes and methods of using the genes in genetically engineered A. succinogenes so as to improve production of chemicals such as succinate, fumarate, malate, 5-aminolevulinate, 2-oxoglutarate, glutamate, and aspartate. The genetically engineered A, succinogenes strains are capable of overexpressing C4 enzymes. The genetically engineered A. succinogenes can have one or more gene knockouts or modifications that inhibit C3 enzymes, The fluxes supplying substrate to the C4 pathway can also be improved in some of the genetically engineered A. succinogenes.
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