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USE OF FLOW CYTOMETRIC ANALYSIS TO OPTIMIZE CELL BANKING STRATEGIES FOR CHO CELLS
USE OF FLOW CYTOMETRIC ANALYSIS TO OPTIMIZE CELL BANKING STRATEGIES FOR CHO CELLS
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机译:使用流式细胞仪分析优化CHO细胞的细胞银行策略
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摘要
Production of biopharmaceuticals from CHO cells requires generation of master-, working- and post-production cell banks of high quality, partly under GMP conditions. An optimal cryopreservation strategy is needed for each new production cell line, particularly with regard to the desire to establish production processes that are completely devoid of serum or even any animal components and to ensure robust thaw performance for reliable production. Here we describe a novel strategy employing flow cytometric (FC) analysis of Annexin V-stained cells for high-throughput characterization of CHO cell banks. Our data show that this method enables evaluation of a cryopreservation procedure just 6 h after thaw.
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