Method of marking and purification of nucleic acids of interest present in a biological sample to be treated in a reaction vessel.

摘要

Method of marking and purification of nucleic acids of interest present in a biological sample to be treated, consisting of: providing a single reaction vessel, introducing into the reaction vessel: wherein: - the biological sample, - at least reagent labeled nucleic acid, it said reagent temperature stable and of formula (0) marked: ** see formula ** - R 1 represents H or an alkyl, aryl or substituted aryl, - R 2 represents a detectable label or at least two detectable labels linked together by at least one multimeric structure, - L is a linking arm comprising a linear chain of at least two covalent bonds and n an integer equal to 0 or 1, - R 3 and R 4 represent independently of each other: H, NO2, Cl, Br, F, I, R 2 - (L) nYX-, oR, SR, NR2, R, NHCOR, CONHR, COOR with R = alkyl or aryl, - a is a linking arm comprising at least one covalent double bond allowing conjugates tion of the diazo group with the aromatic ring and u is an integer from 0 to 2, preferably 0 or 1, and - -YX- represents -CONH-, -NHCO-, -CH 2 O-, CH 2S-. - at least one solid support enabling the adsorption of said nucleic acids, - any necessary marking nucleic acids and / or for the immobilization of said nucleic acids on the support ingredient, - incubating the contents of the reaction vessel, and - isolating the nucleic acids thus labeled.