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Bioassay of environmental toxicity based on ferns (Machine-translation by Google Translate, not legally binding)

机译:基于蕨类植物的环境毒性生物测定(由Google Translate进行的机器翻译,不具有法律约束力)

摘要

Bioassay of environmental toxicity based on ferns. The present invention relates to a method for evaluating the toxicity of a sample based on a) obtaining a suspension of fern spores or gametophytes; b) the incubation of the suspension obtained in a) with the sample whose toxicity is to be evaluated, c) the incubation of the culture obtained in b) in the presence of a tetrazolium sa d) extraction of the tetrazolium salt that has been reduced in step c) as a function of the mitochondrial activity of the spores or gametophytes; and e) determining the amount of reduced tetrazolium salt extracted in d). Also, a kit for the development of the described method is contemplated. (Machine-translation by Google Translate, not legally binding)
机译:基于蕨类植物的环境毒性生物测定。本发明涉及一种基于a)获得蕨类孢子或配子体的悬浮液来评估样品毒性的方法。 b)将a)中获得的悬浮液与要评估其毒性的样品一起温育,c)在四唑盐存在下将b)中获得的培养物温育; d)根据孢子或配子体线粒体活性,提取在步骤c)中还原的四唑盐; e)确定在d)中提取的还原四唑盐的量。同样,考虑了用于开发所述方法的试剂盒。 (通过Google翻译进行机器翻译,没有法律约束力)

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