Site-specific incorporation of cetoaminoacidos in protein.

摘要

Composition comprising an aminoacyl-tRNA synthetase orthogonal (O-RS), where the O-RS preferentially aminoacylates a first orthogonal tRNA (O-tRNA) with acetyl-L-phenylalanine with an efficiency of at least 50% effectiveness a translation system comprising p-acetyl-L-phenylalanine, an O-tRNA of SEQ ID NO .: 21, and synthetase polypeptide comprising an amino acid sequence selected from SEQ ID NO .: 18-20.