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Method for the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides
Method for the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides
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机译:通过掺入非天然核苷酸在体外对核酸进行位点特异性酶标记的方法
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摘要
Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.
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