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THE SELECTIVE QUANTIFICATION METHOD OF AMMONIA OXIDIZING BACTERIA GROUP USING REAL-TIME POLYMERASE CHAIN REACTION

机译:实时聚合酶链反应选择性氧化氨氧化菌群的方法

摘要

A method for measuring selectively and quantitatively anammox bacteria, and a kit for anammox bacteria are provided to analyze the group structure of anammox bacteria. A method for measuring selectively and quantitatively anammox bacteria comprises the steps of preparing the base sequence of a primer or fluorescent material-labeled probe capable of specifically and quantitatively anammox bacteria; carrying out the real time PCR reaction containing the primer or fluorescent material-labeled probe by using the DNA of anammox bacteria as a template; and measuring the fluorescence signal at the previous step to measure the initial sample quantitatively in the reactant.
机译:提供了一种用于选择性和定量测量厌氧细菌的方法,以及用于厌氧细菌的试剂盒,以分析厌氧细菌的组结构。一种选择性和定量地检测厌氧细菌的方法,包括以下步骤:制备能够特异性和定量地消化厌氧细菌的引物或荧光材料标记的探针的碱基序列。以厌氧细菌的DNA为模板,进行包含引物或荧光物质标记探针的实时PCR反应;在前一步骤中测量荧光信号以定量测量反应物中的初始样品。

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