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THE SELECTIVE QUANTIFICATION METHOD OF AMMONIA OXIDIZING BACTERIA GROUP USING REAL-TIME POLYMERASE CHAIN REACTION
THE SELECTIVE QUANTIFICATION METHOD OF AMMONIA OXIDIZING BACTERIA GROUP USING REAL-TIME POLYMERASE CHAIN REACTION
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机译:实时聚合酶链反应选择性氧化氨氧化菌群的方法
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摘要
A method for measuring selectively and quantitatively anammox bacteria, and a kit for anammox bacteria are provided to analyze the group structure of anammox bacteria. A method for measuring selectively and quantitatively anammox bacteria comprises the steps of preparing the base sequence of a primer or fluorescent material-labeled probe capable of specifically and quantitatively anammox bacteria; carrying out the real time PCR reaction containing the primer or fluorescent material-labeled probe by using the DNA of anammox bacteria as a template; and measuring the fluorescence signal at the previous step to measure the initial sample quantitatively in the reactant.
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