PRODUCTION OF NUCLEAR TRANSFER EMBRYOS USING SPERM CYTOSOLIC FACTOR

摘要

A kind of method is arranged to the quality for increasing the incidence of blastocyst and improving blastocyst for cultivating nuclear transfer embryos, to enhance the production efficiency of the animal of clone. A kind of method, includes the following steps: to position for cultivating nuclear transfer embryos and nuclear transfer embryos sperm cytosolic factors and applies voltage to it in cultural mediumcontaining; Mobile nuclear transfer embryos are to the culture medium for culture and cultivate it. The concentration of sperm cytosolic factors is preferably 100-300 glasss/mL.