ATTENUATED DUCK HEPATITIS VIRUS STRAINS, METHOD OF SEPARATING DUCK HEPATITIS VIRUS AND PREPARING PROCESS USING THE SAME

摘要

One separation method of duck hepatitis virus is arranged to produce the vaccine of bulk product cell strain, shows high virus titer, production economy and effective duck virus hepatitis live vaccine and the non-toxic duck hepatitis virus that is directed to is made to have defence capability. One separation method of duck hepatitis virus includes the following steps:: 1) collecting the liver from duck sample infected by duck hepatitis virus, one suspension of manufacture and separate supernatant; 2) inoculation step 1) supernatant liquor in middle duck fetal liver cell, by the duck's egg that manufactures, it is a duck hepatitis virus part and transplants it; 3) culture material of transplanting continuously step 2, freezing and defrosting repeatedly, if cytopathic effect is formed and separates a duck hepatitis virus.