The objective of this study was to characterize the genome structure of duck hepatitis B virus (DHBV) isolated from Hubei brown ducks. The natural carrier rate of DHBV in adult ducks from Hubei area was investigated and the DHBV DNA-positive serum screened out. The complete genome of a DHBV strain was amplified by polymerase chain reaction (PCR) and cloned into T vector and sequenced. The results showed that the carrier rate of DHBV in Hubei brown ducks was 10 %. This strain (GenBank accession number DQ276978) had a genome of 3024 nucleotides with three overlapping open reading frames encoding the surface, core and polymerase proteins respectively. Comparison of the strain with 17 DHBV strains registered in GenBank revealed a homology from 89.3 % to 93.5 % at the nucleotide level. The sequences of the structural and functional domains of these proteins were highly conserved. The strain was found to share more signature amino acids in the polymerase genes with the 'Chinese' DHBV strains than those of the 'Western' country strains. This finding was also corroborated by a phylogenetic tree analysis. Therefore, the DQ276978 might belong to a subtype of the Chinese DHBV strains.
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机译:Untersuchung zur konzentrationsentwicklung in der ernahrungsindustirie in Deutschland。 Ernährungsindustrie(ohneGetränkeindustrie)insgesamt(N.I.C.E。20B)。Herstellung von Fleischkonserven(N.I.C.E.201)。 Herstellung von Obst-undGemüsekonserven(N.I.C.E.203)。Herstellung von Fischkonserven(N.I.C.E.204)=关于德国食品工业集中发展的研究。食品工业(不包括饮料)总计(NICE 20B)。罐头肉的生产(N.I.C.E 201)。水果和蔬菜蜜饯的制造(N.I.C.E.203)。鱼类罐头的制造(N.I.C.E. 204)