KNOCK-OUT VECTOR WHICH IS MANUFACTURED USING CYAN FLUORESCENT PROTEIN REPORTER KNOCK-IN VECTOR AND MANUFACTURING METHOD THEREOF

摘要

PURPOSE: A knock-out vector which is manufactured using a cyan fluorescent protein (CFP) reporter knock-in vector is provided to stably and effectively express a reporter.;CONSTITUTION: A cyan fluorescent protein (CFP) reporter knock-in vector contains a sequence of the sequence number 1. A method for manufacturing the knock-out vector containing the CFP reporter gene comprises: a step of mutating CFP gene sequence containing ATG as an initiation codon; a step of making a promoter sequence of gene to knock-out and polynucleotide; and a step of binding the promoter sequence and polynucleotide at 5'-terminal of ORF of manufactured CFP.;COPYRIGHT KIPO 2010