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PREPARATION METHOD OF RECOMBINANT PROTEIN BY USE OF RNA POLYMERASE ?SUBUNIT AS A FUSION EXPRESSION PARTNER
PREPARATION METHOD OF RECOMBINANT PROTEIN BY USE OF RNA POLYMERASE ?SUBUNIT AS A FUSION EXPRESSION PARTNER
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机译:利用RNA聚合酶β亚单位作为融合表达伴侣的重组蛋白的制备方法
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摘要
Provided is a method for manufacturing a recombinant protein, which can be used for protein medicine and industrial production by using a RNA polymerase alpha subunit as a fusion partner. A method for manufacturing a recombinant protein comprises the following steps of: manufacturing an expression vector by connecting a heterologous protein gene to a RpoA(RNA polymerase alpha subunit) gene as a fusion partner; manufacturing a transformant by employing the expression vector to a host cell; and inducing expression of the recombinant protein by cultivating the transformant. The RpoA gene is written as SEQ ID NO:45.
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机译:本发明提供了通过使用RNA聚合酶α亚基作为融合伴侣而用于蛋白质医学和工业生产的重组蛋白的制造方法。制造重组蛋白的方法包括以下步骤:通过将异源蛋白基因与作为融合伴侣的RpoA(RNA聚合酶α亚基)基因连接来制造表达载体;通过将表达载体用于宿主细胞来制备转化体;通过培养转化子来诱导重组蛋白的表达。 RpoA基因写为SEQ ID NO:45。
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