A preparation method of solubility recombinant protein by use of Chemotaxis protein cheZ as a fusion expression partner

摘要

The present invention is chemotactic protein CheZ (Chemotaxis protein cheZ; CheZ) the fusion expression relates to a partner method of producing a recombinant protein using as, specifically, 1) of the fusion expression partner (the CheZ, and the foreign protein fusion expression partner) than preparing an expression vector connecting the gene, 2) preparing a transformant by introducing the expression vector into a host cell, and 3) culturing the transformant to induce the expression of recombinant proteins to give it Preparation of recombinant proteins comprising the step, and to a recombinant protein produced by the above production method. CheZ of the present invention is how to use the gene of the protein to the protein of E. coli that can maintain its own structural stability under stress environment in fusion expression partners for producing recombinant proteins are soluble and folded with the traditional fusion expression partner ( folding) ability to improve the structural stability of the target protein to maintain and can be expressed at a high yield, can be useful for medical and industrial protein production. ; Chemotactic protein CheZ (Chemotaxis protein CheZ), expression of fusion partner (fusion expression partner), stressful environment