MUTANT ACETOLACTASE SYNTHASE AND METHOD OF PRODUCING BRANCHED L-AMINO ACIDS

摘要

FIELD: biology.;SUBSTANCE: present invention pertains to biotechnology and is a mutant acetolactase synthase of bacteria (AHAS I), in which L-amino acid in positions 17, 30 and/or 33 in a small subunit of natural acetolactase synthase from Escherichia coli is substituted with another L-amino acid or several L-amino acids are integrated in the said position(s), and feedback inhibition with valine in the given subunit is weak. The invention also relates to a DNA fragment, encoding the acetolactase synthase, which is used to transform Escherichia bacteria so as to obtain branched L-amino acid.;EFFECT: invention allows for obtaining branched L-amino acid with high level of efficiency.;17 cl, 8 dwg, 5 tbl, 8 ex