METHOD FOR MAKING SERUM PANEL FOR EVALUATION OF ANTIBODIES TO HEPATITIS B VIRUS

摘要

FIELD: medicine.;SUBSTANCE: invention refers to biotechnology and immunology, namely to immunological diagnostics. There is disclosed a manufacturing technique of serum panels containing antibodies of danger viral infections. For making the serum panel containing various antibodies, within primary screening (by enzyme immunoassay (EIA) and polymerase chain reaction (PCR)), 60 immune-specific bloods serums of HBV-infected persons with positive PCR and 25 donor serums are sampled. The analysis of the EIA and PCR data ensures to establish that the immune-specific serums used in making said panels contain antibodies to HBs, HBc and to HBe antigens. Simultaneously, candidates are sampled as HBV DNA negative and not containing antibodies to HBV antigens. This sample is used to prepare 6 pools of negative samples for the panel.;EFFECT: development of the serum panel which contains all range of antibodies to hepatitis B virus and enables to control clinical effectiveness in treating hepatitis B.;3 tbl, 1 dwg, 2 cl