The present invention relates to novel proteins and enzyme mixtures with the enzymatic activity of a monooxygenase and its use in enzymatic process for the oxidation of methyl groups in aliphatic hydrocarbons. According to the invention, preferably compounds having methyl groups in the branched, aliphatic hydrocarbons, oxidized, which is, in particular, acyclic compounds and / or aliphatic compounds having functional groups or compounds with methyl groups on at the c2 - atom mono - or disubstituted aliphatic hydrocarbons, wherein the substituents can be the same or different. Preferably, the invention relates to a biotechnological process for the oxidation of methyl groups in the branched structures, in which microorganisms which have the desired monooxygenase - activity or, in an aqueous system, are cultured and are converted to corresponding target products even.
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