TARGET NUCLEIC ACID MEASURING METHOD, TARGET NUCLEIC ACID MEASURING APPARATUS, TARGET NUCLEIC ACID MEASURING SYSTEM AND TARGET NUCLEIC ACID MEASURING PROGRAM

摘要

PPROBLEM TO BE SOLVED: To accurately determine the relation of the initial template amount between test samples by using a theoretical expression to which an amount equivalent to an amplification amount of measured nucleic acid is fitted as it is and which accurately reflects actual PCR application efficiency. PSOLUTION: When an amount equivalent to an initial template amount in a test sample is calculated and the relation of initial template amount between test samples is calculated by fitting a thermal cycle number in a nucleic acid amplification reaction performed for a plurality of test samples having the same target nucleic acid and an amount equivalent to an amplification amount of target nucleic acid measured for each thermal cycle number to the theoretical expression, the theoretical expression includes an environmental coefficient as an exponent parameter and the amount equivalent to the initial template amount, and includes at least one parameter among amounts equivalent to a saturation amount upon the target nucleic acid amplification, a reaction acceleration coefficient, and a reaction inhibition coefficient. PCOPYRIGHT: (C)2011,JPO&INPIT