PROBLEM TO BE SOLVED: To provide a target nucleic acid measuring method, a target nucleic acid measuring apparatus, a target nucleic acid measuring system, and a target nucleic acid measuring program, with which the initial template amount can be precisely determined using a theoretical expression that allows to be fitted to the measured detection intensity of the amplification amount of a target nucleic acid in the form of raw data and is capable of precisely reflecting the actual PCR amplification efficiency.SOLUTION: The present invention relates to calculation of the initial template amount in a test sample by fitting the theoretical expression to detection intensity of target nucleic acid corresponding to thermal cycle number, wherein the theoretical expression includes an environmental coefficient as an exponent parameter, a parameter of the initial template amount in the test sample, and terms for internal standard correction and baseline correction of the detection intensity of the amplification amount of the target nucleic acid, and includes at least one parameter among a saturation amount upon the target nucleic acid amplification, a reaction acceleration coefficient, and a reaction inhibition coefficient.
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