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METHOD FOR CREATING MEMBRANE PROTEIN WITH LABELLED N-TERMINAL END, AND CELL HAVING MEMBRANE PROTEIN WITH LABELLED N-TERMINAL END IN ITS OUTER LAYER
METHOD FOR CREATING MEMBRANE PROTEIN WITH LABELLED N-TERMINAL END, AND CELL HAVING MEMBRANE PROTEIN WITH LABELLED N-TERMINAL END IN ITS OUTER LAYER
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机译:带有n末端标记的膜蛋白的制备方法以及在其外层具有n末端标记的膜蛋白的细胞
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摘要
PROBLEM TO BE SOLVED: To provide a method for specifically labelling only a target protein by modifying a specific probe to the target protein, a method for labelling a membrane protein in which its N-terminal end exists extracellularly, a cell having a membrane protein specifically modified by the method, and a method for screening using the cell.;SOLUTION: There is disclosed a method for creating a membrane protein with a labelled N-terminal end, wherein the method is featured by using a labelled molecular probe in which a membrane protein having LPX1X2(G)n sequence (X1 is an optional amino acid, and X2 represents threonine or alanine. n is any one of an integer from 2 to 8.) in a region of N-terminal side and having an N-terminal end extracellularly existing, a peptide transferring enzyme Sortase A, and LPX1X2(G)m sequence (X1 is an optional amino acid, and X2 represents threonine or alanine. m is 1 or 2.) are added.;COPYRIGHT: (C)2010,JPO&INPIT
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机译:解决的问题:为了提供一种通过修饰针对靶蛋白的特异性探针来仅特异性标记靶蛋白的方法,一种标记其N末端存在于细胞外的膜蛋白的方法,一种具有膜蛋白的细胞SOLUTION:公开了一种制备带有标记N末端膜蛋白的方法,其中该方法的特征在于使用了标记的分子探针,其中膜具有LPX 1 Sup> X 2 Sup>(G) n Sub>序列的蛋白质(X 1 Sup>是可选氨基酸,X 2 Sup>代表苏氨酸或丙氨酸,n是2到8的整数之一。)在N末端侧区域中,且N末端在细胞外存在,是一种肽转移酶分选酶A ,并且LPX 1 Sup> X 2 Sup>(G) m Sub>序列(X 1 Sup>是可选氨基酸,X 2 Sup>代表thr氨基酸或丙氨酸。 m是1或2。)添加。;版权:(C)2010,JPO&INPIT
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