PRIMER EVALUATION METHOD, PRIMER EVALUATION PROGRAM, AND REAL-TIME PCR APPARATUS

摘要

PPROBLEM TO BE SOLVED: To provide a primer evaluation method, a primer evaluation program, and a real-time PCR apparatus that enable the design of a primer having temperature resistance at a certain level. PSOLUTION: The primer evaluation method includes obtaining the variation degree of the amplification efficiency obtained when sample sets prepared for the number of temperature conditions that should be made different from each other at an annealing stage are so amplified that a temperature condition at a stage other than the annealing stage is fixed, and submitting the variation degree and a reference value for quality evaluation of a primer, set with respect to the variation degree. PCOPYRIGHT: (C)2010,JPO&INPIT