Primer evaluation method, primer evaluation program and real-time PCR equipment

摘要

A primer evaluation method includes: acquiring a signal indicating time change in an amplification amount obtained when sample sets prepared for the number of temperature conditions that should be made different from each other at an annealing stage in units of target nucleic acids diluted in a stepwise manner are so amplified that a temperature condition at a stage other than the annealing stage is fixed; acquiring a signal indicating initial amounts of the target nucleic acids diluted in a stepwise manner; obtaining amplification efficiency for each of the temperature conditions based on the time change in the amplification amount and the initial amount, and calculating a variation degree of the amplification efficiency; and submitting the variation degree and a reference value for quality evaluation of a primer, set with respect to the variation degree.