TRANSGENIC ANIMALS FOR ANALYZING CYP3A4 CYTOCHROME P450 GENE REGULATION

摘要

PPROBLEM TO BE SOLVED: To provide non-human transgenic animals comprising a reporter construct for producing a detectable amount of a reporter molecule operably linked to a transcriptional regulatory nucleic acid molecule from the human CYP3A4 gene. PSOLUTION: The invention relates to the generation of the non-human transgenic animals comprising the reporter construct for producing the detectable amount of the reporter molecule operably linked to the transcriptional regulatory nucleic acid molecule from the human CYP3A4 gene located between the initiation of transcription site of the gene and a position located 13,000 nucleotides upstream from the site. The invention also relates to the use of these animals for determining the effect of a compound (particularly, but not exclusively, a xenobiotic or steroid) on the regulation of expression of the CYP3A4 gene in a human. PCOPYRIGHT: (C)2010,JPO&INPIT