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Nucleic acid-containing liquid sample analysis for disposable device using a nucleic acid amplification equipment

机译:使用核酸扩增设备的一次性设备的含核酸液体样品分析

摘要

The present invention is directed to (1) disposable sample holding and processing device which is dimensioning so as to be operated in a nucleic acid containing liquid sample for analyzing nucleic acid amplification apparatus according to nucleic acid amplification techniques. The device (1), (44), the path must chambers designed to perform the capture of nucleic acid (1) in the process of the amplification and detection unit (15, 16) the melting chamber also (3) including. Binding chamber (15), the amplification chamber (16), is connected binding chamber (15) through (44) the fluid pathway also includes a (14) solid phase for immobilizing the components of the sample to be analyzed there. Device (1), (44), the binding chamber (15) and the amplification chamber (16), placed side body (42) to (50) on at least one path also includes a (42) rigid body, (amplification chamber (15) or (44), the binding chamber path (16) has been placed on it), each side of these (50), is covered by (41) at least one wall, Also, when actuated by a nucleic acid amplification apparatus, aspects of these (50), apparatus (1) is a vertical plane substantially. [Selection Figure Figure 4
机译:本发明涉及(1)一次性样品保持和处理装置,其尺寸设计成可在含核酸的液体样品中操作,以用于根据核酸扩增技术分析核酸扩增装置。装置(1),(44),路径必须为腔室,该腔室被设计为在还包括熔解室(3)的扩增和检测单元(15、16)的过程中执行核酸(1)的捕获。结合室(15),即扩增室(16),通过(44)与结合室(15)相连。流体通道还包括(14)固相,用于将待分析样品的组分固定在那里。在至少一条路径上放置侧体(42)至(50)的装置(1),(44),装订室(15)和扩增室(16)也包括(42)刚性体(扩增室) (15)或(44),已将结合室路径(16)放在其上),这些(50)的每一侧均被(41)至少一个壁覆盖,此外,当通过核酸扩增致动时这些装置(50)的各个方面,装置(1)基本上是垂直平面。 [选择图图4

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