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Nucleotide sequence determination method, nucleotide sequence determination system and nucleotide sequence determination program

机译:核苷酸序列确定方法,核苷酸序列确定系统和核苷酸序列确定程序

摘要

A method for determining nucleotide sequence encompasses (a) injecting a solution containing a sample nucleic acid into a chip cartridge, (b) detecting first detection, second detection, and control signals through electrodes on the chip cartridge, (c) calculating a first difference between a mean value of the control signals and a mean value of the first detection signals, and dividing the first difference by the mean value of the control signals to define X, (c) calculating a second difference between the mean value of the control signals and a mean value of the second detection signals, and dividing the second difference by the mean value of the control signals to define Y, (d) calculating an angle between a positive X-axis and a vector from origin to a point (X, Y), and (d) comparing the angle with angle judgment criteria so as to identify a genotype of the sample nucleic acid.
机译:确定核苷酸序列的方法包括:(a)将包含样品核酸的溶液注入芯片盒中;(b)通过芯片盒上的电极检测第一检测信号,第二检测信号和控制信号;(c)计算第一差异在控制信号的平均值和第一检测信号的平均值之间,将第一差除以控制信号的平均值以定义X,(c)计算控制信号的平均值之间的第二差(d)计算正X轴与从原点到点的矢量之间的夹角(X,并用第二检测信号的平均值除以控制信号的平均值以定义Y)。 Y),以及(d)将角度与角度判断标准进行比较,以识别样品核酸的基因型。

著录项

  • 公开/公告号JP4516107B2

    专利类型

  • 公开/公告日2010-08-04

    原文格式PDF

  • 申请/专利权人 株式会社東芝;

    申请/专利号JP20070327737

  • 发明设计人 本郷 禎人;

    申请日2007-12-19

  • 分类号C12Q1/68;G01N27/416;G01N27/327;G01N27/26;C12M1/00;C12N15/09;

  • 国家 JP

  • 入库时间 2022-08-21 18:58:49

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