Fusion proteins comprising a bacteriophage coat protein and single-chain T-cell receptor

摘要

(57) novel fusion protein comprising a single-chain T-cell receptor and the bacteriophage coat proteins, and, [Abstract] The present invention relates to the use of the complex. Viewed from one aspect, the invention is directed to soluble fusion proteins comprising a bacteriophage coat protein that is covalently linked to a single chain T cell receptors, single chain T cell receptors described above, the peptide linker It is configured to include covalently linked to V- beta chain by sequencing the V- alpha gene. Soluble fusion protein according to the present invention are useful in a variety of applications. For example, it is used to screen for the identification and isolation of ligands to build a bacteriophage library for presenting 1) a single chain T cell receptor binds to the single-stranded T-cell receptor and the library in that It, and methods of 2) the fusion protein, and isolating the single chain T cell receptors having functionality and sufficient solubility, and the like.