FUSION PROTEINS COMPRISING BACTERIOPHAGE COAT PROTEIN AND A SINGLE-CHAIN T CELL RECEPTOR

摘要

The present invention relates to novel fusion proteins comprising a bacteriophage coat protein and a single-chain T cell receptor and uses of such complexes. In one aspect, the invention relates to soluble fusion protein comprising a bacteriophage coat protein covalently linked to a single-chain T cell receptor which comprises a V-alpha gene covalently linked to a V-beta chain by a peptide linker sequence. The soluble fusion proteins of the invention are useful for a variety of applications including: 1) making a bacteriophage library for displaying single-chain T cell receptors for use in screens for identification and isolation of ligands that bind signel-chain T cell receptors, and 2) methods for isolating soluble and fully functional single-chain T cell receptors from the fusion proteins.