Ethylene Signalling genes in maize and its Modulation to enhance stress Tolerance in Plants

摘要

It provides Nucleic Acids isolated from maize ELN3 ERF3, EBF1, EBF2, ein5 that are associated with the Ethylene Signalling in plants and their encoded Proteins methods and compositions that relate to the alteration of ethylene sensitivity in plants. Recombinant expression cassettes also host cells, transgenic plants, Seeds and Antibody compositions.Claim 1: an isolated Nucleic Acid comprising a member selected from the group consisting of: (a) a polynucleotide which is at least 90% Sequence identity, as determined by the Blast algorithm 2.0 under default parameters with a polynucleotide encoding a polypeptide selected from the group consisting of SEQ ID Nos: 10, 8, 6 or 4; (b) a polynucleotide that encodes a polypeptide selected from the group consisting of SEQ ID Nos: 10, 8,6 or 4; (c) a polynucleotide Amplification of a Nucleic Acid Library of Zea mays using primers? That hybridize selectively in stringent conditions of hybridization within a Gene loci selected from the group consisting of SEQ ID Nos: 9, 7, 5.And 3; (d) a polynucleotide that stringent hybridization conditions selectively in Hybrid and washed in 2x SSC 50 c, in a Gene selected from the group consisting of SEQ ID Nos: 9, 7, 5 and 3; (e) a Gene selected from the group consisting of SEQ ID Nos: 9, 7, 5 And 3; (f) a polynucleotide which is complementary to a polynucleotide of (a), (b), (c),(d) or (e); and (g) a polynucleotide comprising at least 25 contiguous nucleotides of a polynucleotide of (a), (b), (c), (d), (e) and (f).