Preparation of active Human lysosomal sulfatase enzymes and highly phosphorylated and its uses

摘要

Also provided are compositions comprising Active and highly phosphorylated lysosomal sulfatase Enzymes, their pharmaceutical compositions, Methods for producing and PURIFYING these lysosomal sulfatase enzymes and compositions and their use in the diagnosis, prophylaxis and Treatment Diseases and conditionsEven particularly for Lysosomal Storage diseases that are caused or are associated with a deficiency of the lysosomal enzyme - sulfatase.Claim 1: a Recombinant human lysosomal sulfatase Enzyme or its fragment, derived Biologically Active Variant or Mutant Cho Cell produced by a group of end3 complementation or its derivative, the CHO Cell end3 complementation group or its derivative expresses the factor The Recombinant human sulfatase 1 modifier (SUMF1)And where such lysosomal sulfatase Enzyme or its Variant or fragment, Mutant, Biologically Active derivative has a high Activation level and a high level of phosphorylation.