GENETICALLY MODIFIED ASPERGILLUS ORYZAE AND A PLASMID TO USE IN ASPERGILLUS FOR PRODUCTION OF ALPHA-AMYLASE AND PROCESSES THEREOF.

摘要

Genetically modified A.oryzae (MTCC 5177) for better production of amylase having sequence as herein described from locally available GRAS fungus A.oryzae E212 from the plasmid pAAC3 (9) obtained from cloning of 2.8213Kbpamylase fragment having a blunt end BamH1 side and a staggered end EcoR1 at both the ends derived from plasmid pSPK14 (1) and the 5Kbf"acetamidase gene of A. nidulans derived from p3SR2 (4) having a EcoR1 blunt end and Sal1 staggered end at both the ends with linearized plasmid pGEM 3Z having Sal1 and EcoR1 sides at both the ends and process thereof.