Transgenic plants expressing cellulase for autohydrolysis of cellulose components and method for production of soluble sugar

摘要

Disclosed is a recombinant cDNA encoding a fusion protein comprising chloroplast-targeting peptide, full length cellulase and cellulose binding domain sequentially, wherein the cellulase is cellulase5 originated from Ruminococcus albus, and the cellulose binding domain is originated from Xylanase A of Clostridium stercorarium. The cDNA can be used to produce plants expressing cellulase for autohydrolysis. Methods for the production of soluble sugars comprising the induction of autohydrolysis of the cellulose are also disclosed.