DETECTION OF BACTERIA OF THE LACTIC ACID HISTAMINE PRODUCERS THROUGH CHAIN REACTION OF THE REAL TIME QUANTITATIVE POLYMERASE (QRT-PCR) AND ITS APPLICATIONS.

摘要

Lactic acid bacteria detectionhistamine producers by chain reaction of theReal-time quantitative polymerase (QRT-PCR) andYour applications;The present invention describes a method of quantitative amplification in real time of the hdcA gene by QRT-PCR, based on the use of a pair of oligonucleotide primers and Taqman probe specific gene HDCA Lb. buchneri NIZO 301 under suitable conditions of amplification of the double stranded fragment flanked by said oligonucleotides. This procedure identifies potentially histamine-producing bacteria that allow decisions to be made in the field of food safety, preferably in the dairy sector or in the wine sector, both to ensure the characterization of the strains that may be part of an initiator culture. as to control the processes of fermentation once initiated and of foods already elaborated. The advantages of the proposed procedure are, in addition to specificity and simplicity, very high sensitivity and speed.