ISOMETRIC PRIMER EXTENSION METHOD AND KIT FOR DETECTION AND QUANTIFICATION OF SPECIFIC NUCLEIC ACID

摘要

A method for detecting and/or quantifying a target DNA or RNA present in asample by a isometric primer extension method is disclosed. The methodincludescarrying out a primer extension reaction in the absence of a free nucleotideso that theprimer extension reaction is stopped where the absent nucleotide would havebeeninserted. Thus, as the amount of incorporation of a labeled nucleotide on theprimerextended product is detected, the amount of the target RNA or DNA in thesample ismeasured.