EXPRESSION AND SECRETION VECTOR FOR HUMAN INTERFERON ALPHA AND PROCESS FOR PRODUCING HUMAN INTERFERON ALPHA BY EMPLOYING SAME

摘要

Disclosed in this invention are: an expression vector for the secre-tiveproduction of human interferon alpha (hIFNa) comprising a polynucleotideencoding a modified E. coli thermostable enterotoxin II signal sequence and apolynucleotide encoding hIFNa) ligated to the 3'-end thereof; a microorganismtransformed with the expression vector; and a process for secretivelyproducinghuman interferon by culturing the microorganism, said process being capable ofsecreting a soluble form of active hIFN.alpha.), which does not contain anadditionalmethionine residue at its N-terminal, into the periplasm of an E. coli cell.