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METHOD FOR INDIRECTLY EXTRACTING NON-CULTIVABLE DNA ORGANISMS AND DNA OBTAINABLE BY SAID METHOD

机译:SAID法间接提取不可培养的DNA有机体和可利用的DNA的方法

摘要

process for the extraction of target dna (more than 300 kb in sizenon culturable contentin an environmental sample, which is ground in a liquid medium.the sample, we get the product is obtained.the rest of the product between the agencies by sedimentation on pillowdensity greater than 1, we get the isolated organismson the surface of the pillow is included in a block are extracted from agarose gel.the dna in the agarose block analysis, we take thethe block in an agarose gel and the electrophoresis is subjected to at least one byalternating field, finally, we get the dna strandthe extracts.
机译:提取目标dna的过程(大小超过300 kb不可培养的内容在环境样品中,该样品在液体介质中研磨。样品,我们得到的产品就是获得的。其余产品在机构之间通过在枕头上沉淀密度大于1,我们得到了孤立的生物枕头表面上的一个块体是从琼脂糖凝胶中提取的。琼脂糖块分析中的dna,我们将将其在琼脂糖凝胶中进行电泳,然后至少电泳一次最后,我们得到了dna链提取物。

著录项

  • 公开/公告号CA2407270C

    专利类型

  • 公开/公告日2010-10-19

    原文格式PDF

  • 申请/专利权人 LIBRAGEN;

    申请/专利号CA20012407270

  • 发明设计人 TRAN VAN VAN;NALIN RENAUD;ROBE PATRICK;

    申请日2001-04-03

  • 分类号C12N15/10;C07H21/04;G01N27/447;

  • 国家 CA

  • 入库时间 2022-08-21 18:42:51

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