首页> 外国专利> METHOD FOR PRODUCING HIGH CONCENTRATE LACTIC ACID BACTERIA WITH MEMBRANE BIOREACTOR AND FREEZE-DRIED, LACTIC ACID BACTERIA POWDER

METHOD FOR PRODUCING HIGH CONCENTRATE LACTIC ACID BACTERIA WITH MEMBRANE BIOREACTOR AND FREEZE-DRIED, LACTIC ACID BACTERIA POWDER

机译:膜生物反应器和冷冻干燥乳酸菌粉制备高浓度乳酸菌的方法。

摘要

The present invention relates to a method for producing lactic acid bacteria of a high concentration continuously using a membrane bioreactor. Specifically, the present invention relates to a method for producing lactic acid bacteria of a high concentration in a membrane bioreactor that comprises the steps of cultivating lactic acid bacteria in a membrane bioreactor including a membrane for product separation and a medium supply apparatus; supplying culture media to the bioreactor through the medium supply apparatus; continuously separating and discharging culture filtrate through the membrane for product separation; and recycling lactic acid bacteria continuously to the bioreactor. Additionally, the present invention relates to a method for producing lactic acid bacteria powder by freeze drying the bacteria produced in the membrane bioreactor using a freeze drying preservative composition. The lactic acid bacteria obtained through the membrane bioreactor is obtained in pellet form with a centrifugal separator, and is subjected to freeze-drying using the composition for freeze drying preservative containing certain amounts of trehalose, maltodextrin, starch and sodium carboxy methyl cellulose to form lactic acid powder. The lactic acid bacteria that have been converted into powder by such method exhibit superior stability chemically and physically compared to lactic acid bacteria powder that has been subjected to simple freeze drying.
机译:本发明涉及一种使用膜生物反应器连续生产高浓度乳酸菌的方法。具体地,本发明涉及在膜生物反应器中生产高浓度乳酸菌的方法,该方法包括以下步骤:在膜生物反应器中培养乳酸菌,该膜生物反应器包括用于产品分离的膜和介质供应装置;通过培养基供给装置向生物反应器供给培养基;通过膜连续分离和排出培养滤液以分离产物;并将乳酸菌不断循环到生物反应器中。另外,本发明涉及通过使用冷冻干燥的防腐剂组合物冷冻干燥在膜生物反应器中产生的细菌来生产乳酸菌粉末的方法。通过膜生物反应器获得的乳酸菌是通过离心分离机以小球形式获得的,并使用含有一定量海藻糖,麦芽糊精,淀粉和羧甲基纤维素钠的防腐剂冷冻干燥用组合物进行冷冻干燥以形成乳酸。酸粉。与已经进行简单冷冻干燥的乳酸菌粉末相比,通过这种方法转化为粉末的乳酸菌在化学和物理上显示出优异的稳定性。

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