PRIMERS FOR DETECTING NOROVIRUS AND METHOD FOR IDENTIFYING AND DETECTING NOROVIRUS GENES COMPRISING THE SAME

摘要

PURPOSE: a kind of detection norwalk virus is provided to specific reply norwalk virus, and which causes the acute gastroenteritiss of clinical symptoms. ;CONSTITUTION: primer RT-PCR one-step method includes sequence serial number 1 and 2,4 and 5 for detecting norwalk virus gene. Contain sequence number 2,3,5 and 6 in heminested PCR primer sequence. A kind of One step RT-PCR kit includes primer, archaeal dna polymerase and buffer. A kind of detection norwalk virus genotype includes: the step of extracting viral RNA, from sample; One step carries out RT-PCR viral RNAs and uses the primer sets; One step carries out the primer of the PCR in heminested PCR product purpose; The PCR product of laminating step is through electrophoresis; With determine specific reaction, for 1 type and 2 types of norovirus. ;The 2010 of copyright KIPO submissions