RT-PCR METHOD USING N-PRIMER AND KIT FOR RT-PCR COMPRISING N-PRIMER

摘要

PURPOSE: A method and kit of RT-PCR using N-primer is provided to improve primer specificity and minimize non-specific PCR. CONSTITUTION: An RT-PCR method using N-primer comprises: a step of annealing RNA as a template with the N-primer to synthesize first strand cDNA; a step of adding forward primer and reverse primer with cDNA; a step of adding N-primer, forward primer, reverse primer, reveretranscriptase, thermostable polymerase and reaction buffer to perform RT-PCR; The length of N-primer is 20-30 nucleotides. The RNA is derived from plant virus. A kit for RT-PCR contains N-primer and reagent for RT-PCR.