PCR PRIMER SET FOR DETECTING KASHMIR BEE VIRUS(KBV) OF HONEY BEE AND A METHOD FOR DETECTING KASHMIR BEE VIRUS USING THE SAME

摘要

PURPOSE: A PCR primer set which is able to detect a specific gene fragment of kashmir bee virus(KBV) of honey bee is provided to accurately and sensitively detect KBV.;CONSTITUTION: A PCR primer set for detecting Kashmir bee virus(KBV) comprises KIRT-F primer and KIRT-R primer. A forward primer KIRT-F has 5'-GGGCTTTATAGCTCATTTGATGG-3' of sequence number 1. The reverse primer KIRT-R has 5'-CTTGCGGACAGTTTTACTCCA-3' of sequence number 2. The detection of KBV infection is performed by PCR using the PCR primer set and confirming PCR product.;COPYRIGHT KIPO 2010