Method for isolating the useful flavonoid-rich fraction and novel flavonoid compound from the aerial part of Sageretia theezans

摘要

The present invention is directed to a method of separating the flavonoid substances: dry and fry the chopped above-ground tree homology, After degreasing bath comprising: a first step of extraction with chloroform as a 60% aqueous acetone solution again (S10); The first step (S10) re-extracted acetone extract from the crude extract hexane, ethyl acetate, butanol, and the second step of the solvent fractionation with water (S20); And the fraction obtained by eluting the extract of the ethyl acetate fraction was fractionated in the second step (S20) and irradiated with a thin layer chromatography (TLC) novel flavonoid (flavonoid) 7- O - methyl-mail is Citrine (7- O -methyl mearnsitrin) to obtain a third (S30); jidoe comprises a, the third step (S30) is Sephadex (sephadex) LH- the ethyl acetate fraction 20 obtained by elution in the column chromatography in 30% to 100% methanol, and the third step (S30) of the -OCH3 group attached to rhamnose (rhamnose) glycosides in para position of the B ring flavonoid 7- O -. methyl-mail is citrine (7- O -methyl mearnsitrin) and (2), wherein the generating a ; As a result, food additives, functional health Food, circulatory disease prevention and treatment, and other medicines and is used as the main raw material parts can be reduced activation of high-value material development and related industries of native plants in the country.